I can reproduce the error with
clusterExperiment/vignettes master$ R_DEFAULT_PACKAGES= LC_COLLATE=C R -f clusterExperimentTutorial.R
leading to
## ----recallRSEC------------------------------------------------------------
rsecFluidigm<-RSEC(rsecFluidigm,isCount=TRUE,combineProportion=0.6,mergeMethod="JC",mergeCutoff=0.05)
Error in validObject(.Object) :
invalid class "ClusterExperiment" object: merge_nodeMerge must be data.frame with 4 columns and column names equal to: 'Node','Contrast','isMerged','mergeClusterId'
Calls: RSEC ... .local -> new -> initialize -> initialize -> validObject
Execution halted
and then for work inside R
clusterExperiment/vignettes$ R_DEFAULT_PACKAGES= LC_COLLATE=C R
Bioconductor version 3.6 (BiocInstaller 1.27.7), ?biocLite for help
source("clusterExperimentTutorial.R", echo=TRUE, max=Inf)
Does that set you down the right path?
Martin