[Bioc-devel] VariantAnnotation: Convertion from VCF to VRanges for CG example VCF
Hi Julian, I'm looking into this. It's likely that the chr7 file has an invalid header for the AD variable (which should be appropriately handled by readVcf()). I'll let you know when it's resolved. Valerie
On 12/07/2013 06:51 AM, Julian Gehring wrote:
Hi,
I tried to import example VCFs from 'VariantAnnotation' and convert it
to a 'VRanges' object. While this works fine for the 'chr22.vcf.gz', it
fails for the 'chr7-sub.vcf.gz' VCF:
#+BEGIN_SRC R
library(VariantAnnotation)
f = system.file("extdata", "chr7-sub.vcf.gz", package =
"VariantAnnotation", mustWork = TRUE)
vcf = readVcf(f, "hg19")
vr = as(vcf, "VRanges")
#+END_SRC
results in:
#+BEGIN_EXAMPLE
Error in validObject(.Object) :
invalid class ?SummarizedExperiment? object: 'rowData' length differs
from 'assays' nrow
#+END_EXAMPLE
The traceback returns:
#+BEGIN_EXAMPLE
16: stop(msg, ": ", errors, domain = NA)
15: validObject(.Object)
14: initialize(value, ...)
13: initialize(value, ...)
12: new("SummarizedExperiment", exptData = exptData, rowData = rowData,
colData = colData, assays = assays, ...)
11: .local(assays, ...)
10: SummarizedExperiment(assays = geno, rowData = rowData, colData =
colData,
exptData = exptData)
9: SummarizedExperiment(assays = geno, rowData = rowData, colData =
colData,
exptData = exptData)
8: initialize(value, ...)
7: initialize(value, ...)
6: new(class, SummarizedExperiment(assays = geno, rowData = rowData,
colData = colData, exptData = exptData), fixed = fixed, info =
info,
...)
5: VCF(rowData = rdexp, colData = colData(x), exptData = exptData(x),
fixed = fexp, info = iexp, geno = gexp, ..., collapsed = FALSE)
4: expand(from)
3: expand(from)
2: asMethod(object)
1: as(vcf, "VRanges")
#+END_EXAMPLE
This occurs both with bioc-release and bioc-devel (all packages up to
date 2013-12-07).
Best wishes
Julian
Valerie Obenchain Program in Computational Biology Division of Public Health Sciences Fred Hutchinson Cancer Research Center 1100 Fairview Ave. N, M1-B155 P.O. Box 19024 Seattle, WA 98109-1024 E-mail: vobencha at fhcrc.org Phone: (206) 667-3158 Fax: (206) 667-1319