[Bioc-devel] readGAlignmentPairs function broken?
Hi Leonard, What happened between GenomicAlignments 0.99.8 and GenomicAlignments 0.99.10 is that I modified readGAlignmentPairsFromBam() to use the new pairing algo (implemented in scanBam()) instead of the pairing algo implemented by findMateAlignment(): ------------------------------------------------------------------------ r84106 | hpages at fhcrc.org | 2013-12-09 23:49:43 -0800 (Mon, 09 Dec 2013) | 3 lines Modify readGAlignmentPairsFromBam() to use scanBam(BamFile(asMates=TRUE), ...) instead of findMateAlignment() for the pairing. ------------------------------------------------------------------------ The new pairing algo (implemented by Val and Martin) is fast and memory efficient, and, last but not least, supports 'yieldSize' for reading the BAM file by chunk. The same pairing algo is also used by readGAlignmentsListFromBam(). readGAlignmentPairsFromBam() actually calls readGAlignmentsListFromBam() and then turns the returned GAlignmentsList object into a GAlignmentPairs object. I need to do some timings but I suspect this transformation is taking a little bit too long and there might be room for optimization (like e.g. avoiding the GAlignmentsList intermediate representation). I'll keep you updated. Thanks, H.
On 12/19/2013 12:58 PM, Leonard Goldstein wrote:
Hi Herv? Thanks for fixing this problem. It works fine now, however I did notice a drop in performance. For example reading in chromosome 1 can take about twice as long as previously (see below). Is this something that can be avoided? Many thanks again Leonard -- GenomicAlignments 0.99.8
param <- ScanBamParam(which = GRanges("1", IRanges(1, 249250621)))
system.time(readGAlignmentPairs(file, param = param))
user system elapsed 161.282 7.812 169.442
system.time(readGAlignmentPairs(file, param = param))
user system elapsed 91.614 7.256 99.065
system.time(readGAlignmentPairs(file, param = param))
user system elapsed 89.285 7.461 96.940
sessionInfo()
R Under development (unstable) (2013-12-03 r64376) Platform: x86_64-unknown-linux-gnu (64-bit) locale: [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C [3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8 [5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8 [7] LC_PAPER=en_US.UTF-8 LC_NAME=C [9] LC_ADDRESS=C LC_TELEPHONE=C [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C attached base packages: [1] parallel stats graphics grDevices utils datasets methods [8] base other attached packages: [1] GenomicAlignments_0.99.8 Rsamtools_1.15.15 Biostrings_2.31.5 [4] GenomicRanges_1.15.17 XVector_0.3.5 IRanges_1.21.17 [7] BiocGenerics_0.9.2 loaded via a namespace (and not attached): [1] bitops_1.0-6 stats4_3.1.0 zlibbioc_1.9.0
GenomicAlignments 0.99.10
param <- ScanBamParam(which = GRanges("1", IRanges(1, 249250621)))
system.time(readGAlignmentPairs(file, param = param))
user system elapsed 265.624 8.812 274.990
system.time(readGAlignmentPairs(file, param = param))
user system elapsed 249.724 7.177 257.399
system.time(readGAlignmentPairs(file, param = param))
user system elapsed 247.476 6.648 254.621
sessionInfo()
R Under development (unstable) (2013-12-03 r64376) Platform: x86_64-unknown-linux-gnu (64-bit) locale: [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C [3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8 [5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8 [7] LC_PAPER=en_US.UTF-8 LC_NAME=C [9] LC_ADDRESS=C LC_TELEPHONE=C [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C attached base packages: [1] parallel stats graphics grDevices utils datasets methods [8] base other attached packages: [1] GenomicAlignments_0.99.10 Rsamtools_1.15.15 [3] Biostrings_2.31.5 GenomicRanges_1.15.17 [5] XVector_0.3.5 IRanges_1.21.17 [7] BiocGenerics_0.9.2 loaded via a namespace (and not attached): [1] bitops_1.0-6 stats4_3.1.0 zlibbioc_1.9.0
On Thu, Dec 19, 2013 at 11:52 AM, Herv? Pag?s <hpages at fhcrc.org> wrote:
Hi Leonard, This should be fixed in GenomicAlignments 0.99.10, which will become available thru biocLite() in the next 24 hours or so. In the mean time you can grab it directly from svn: https://hedgehog.fhcrc.org/bioconductor/trunk/madman/Rpacks/GenomicAlignments Please let me know if you still run into problems with this. Thanks! H. On 12/19/2013 10:41 AM, Leonard Goldstein wrote:
Hi Herv? You probably spotted this already but it looks like the problem is introduced between GenomicAlignments revisions r84052 (0.99.8) and r84106 (0.99.9) Best wishes Leonard On Wed, Dec 18, 2013 at 5:25 PM, Leonard Goldstein <goldstel at gene.com> wrote:
Dear list, There seems to be a problem with the readGAlignmentPairs function: Querying genomic regions without any alignments using the which argument results in an error -- see (1) below. Reading in a whole chromosome takes indefinitely (or at least much longer than in previous versions) -- see (2) below. I suspect these issues are not specific to the BAM files I am working with but can provide test data if required. Many thanks for your help. Leonard -- (1) Attempts to read an empty region results in an error.
gr <- GRanges("22", IRanges(1000000, 2000000))
param <- ScanBamParam(which = gr)
readGAlignmentPairs(file, param = param)
Error in `elementMetadata<-`(x, ..., value = value) :
replacement 'elementMetadata' value must be a DataTable object or NULL
sessionInfo()
R Under development (unstable) (2013-12-03 r64376) Platform: x86_64-unknown-linux-gnu (64-bit) locale: [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C [3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8 [5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8 [7] LC_PAPER=en_US.UTF-8 LC_NAME=C [9] LC_ADDRESS=C LC_TELEPHONE=C [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C attached base packages: [1] parallel stats graphics grDevices utils datasets methods [8] base other attached packages: [1] GenomicAlignments_0.99.9 Rsamtools_1.15.15 Biostrings_2.31.5 [4] GenomicRanges_1.15.17 XVector_0.3.5 IRanges_1.21.16 [7] BiocGenerics_0.9.2 BiocInstaller_1.13.3 loaded via a namespace (and not attached): [1] bitops_1.0-6 stats4_3.1.0 tools_3.1.0 zlibbioc_1.9.0
... but works fine with previous version
gr <- GRanges("22", IRanges(1000000, 2000000))
param <- ScanBamParam(which = gr)
readGAlignmentPairs(file, param = param)
GAlignmentPairs with 0 alignment pairs and 0 metadata columns:
seqnames strand : ranges -- ranges
<Rle> <Rle> : <IRanges> -- <IRanges>
---
seqlengths:
1 2 3 ... GL000247.1 GL000248.1 GL000249.1
249250621 243199373 198022430 ... 36422 39786 38502
sessionInfo()
R version 3.0.0 (2013-04-03) Platform: x86_64-unknown-linux-gnu (64-bit) locale: [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C [3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8 [5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8 [7] LC_PAPER=C LC_NAME=C [9] LC_ADDRESS=C LC_TELEPHONE=C [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C attached base packages: [1] parallel stats graphics grDevices utils datasets methods [8] base other attached packages: [1] Rsamtools_1.14.2 Biostrings_2.30.1 GenomicRanges_1.14.4 [4] XVector_0.2.0 IRanges_1.20.6 BiocGenerics_0.8.0 loaded via a namespace (and not attached): [1] bitops_1.0-6 stats4_3.0.0 zlibbioc_1.8.0 (2) Use of the which argument covering chromosome 22 takes under one minute with an earlier version of readGAlignmentPairs
gr <- GRanges("22", IRanges(1, 51304566))
param <- ScanBamParam(which = gr)
system.time(gap <- readGAlignmentPairs(file, param = param))
user system elapsed
45.887 0.256 46.168
sessionInfo()
R version 3.0.0 (2013-04-03) Platform: x86_64-unknown-linux-gnu (64-bit) locale: [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C [3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8 [5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8 [7] LC_PAPER=C LC_NAME=C [9] LC_ADDRESS=C LC_TELEPHONE=C [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C attached base packages: [1] parallel stats graphics grDevices utils datasets methods [8] base other attached packages: [1] Rsamtools_1.14.2 Biostrings_2.30.1 GenomicRanges_1.14.4 [4] XVector_0.2.0 IRanges_1.20.6 BiocGenerics_0.8.0 loaded via a namespace (and not attached): [1] bitops_1.0-6 stats4_3.0.0 zlibbioc_1.8.0
... but at least twenty times as long with the current version.
gr <- GRanges("22", IRanges(1, 51304566))
param <- ScanBamParam(which = gr)
system.time(gap <- readGAlignmentPairs(file, param = param))
^C Timing stopped at: 1108.041 35.998 1144.006
sessionInfo()
R Under development (unstable) (2013-12-03 r64376) Platform: x86_64-unknown-linux-gnu (64-bit) locale: [1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C [3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8 [5] LC_MONETARY=en_US.UTF-8 LC_MESSAGES=en_US.UTF-8 [7] LC_PAPER=en_US.UTF-8 LC_NAME=C [9] LC_ADDRESS=C LC_TELEPHONE=C [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C attached base packages: [1] parallel stats graphics grDevices utils datasets methods [8] base other attached packages: [1] GenomicAlignments_0.99.9 Rsamtools_1.15.15 Biostrings_2.31.5 [4] GenomicRanges_1.15.17 XVector_0.3.5 IRanges_1.21.16 [7] BiocGenerics_0.9.2 loaded via a namespace (and not attached): [1] bitops_1.0-6 stats4_3.1.0 zlibbioc_1.9.0
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-- Herv? Pag?s Program in Computational Biology Division of Public Health Sciences Fred Hutchinson Cancer Research Center 1100 Fairview Ave. N, M1-B514 P.O. Box 19024 Seattle, WA 98109-1024 E-mail: hpages at fhcrc.org Phone: (206) 667-5791 Fax: (206) 667-1319
Herv? Pag?s Program in Computational Biology Division of Public Health Sciences Fred Hutchinson Cancer Research Center 1100 Fairview Ave. N, M1-B514 P.O. Box 19024 Seattle, WA 98109-1024 E-mail: hpages at fhcrc.org Phone: (206) 667-5791 Fax: (206) 667-1319