[Bioc-devel] BiomartGeneRegionTrack question
Hi,
On 07/27/2016 04:49 AM, Hahne, Florian wrote:
Valerie, I took a somewhat closer look at this, and I think that a mapping between Ensembl genome version and UCSC genome identifiers is all that is needed from the Bioconductor side.
OK, sounds good. Lori (lori.shepherd at roswellpark.org) and I will be
working on this together. We'll probably implement a mapGenomes()
function something like -
UCSC -> Ensembl is (possibly) one to many and this call
mapGenomes("dasNov3", "Ensembl")
would return a vector:
c("ARMA", "Dasnov3.0")
Ensembl -> UCSC is one to one and this call
mapGenomes("ARMA", "UCSC")
would return a string:
"dasNov3"
Is returning the genome name sufficient or do you also want a date or
other info?
I can figure out a way to identify the relevant Ensembl archive to load during Gviz package build.
Biomart provides the version information of all data sets via the listDatasets() function in the form of a data.frame:
head(ds)
dataset description
1 oanatinus_gene_ensembl Ornithorhynchus anatinus genes (OANA5)
2 cporcellus_gene_ensembl Cavia porcellus genes (cavPor3)
3 gaculeatus_gene_ensembl Gasterosteus aculeatus genes (BROADS1)
4 itridecemlineatus_gene_ensembl Ictidomys tridecemlineatus genes (spetri2)
5 lafricana_gene_ensembl Loxodonta africana genes (loxAfr3)
6 choffmanni_gene_ensembl Choloepus hoffmanni genes (choHof1)
version
1 OANA5
2 cavPor3
3 BROADS1
4 spetri2
5 loxAfr3
6 choHof1
As you can see, the species is somewhat stored in the dataset column, but not in a standard term. The genome or assembly version is stored in the version column. With that information and the table provided here (https://genome.ucsc.edu/FAQ/FAQreleases.html) it should be fairly straight forward to set up a manual mapping. If you do not want to go through all the old Biomart archives you can get a complete listing from this table on the ENSEMBL web site: http://www.ensembl.org/info/website/archives/assembly.html
I have already done this exercise with the tables in the Gviz package, and could provide a current version with the relevant information.
I think the tables in Gviz are a good place to start and would like to keep some version of that format in GenomeInfoDb. If you can consolidate them into one we (Lori and I) can go through the process of updating with your help. Let us know (off-line) when you've got the table ready or if you think collapsing to one isn't a good idea after all. Thanks. Valerie
Mappings from UCSC genome to ENSEMBL versions do not have to be unique since the latter are typically down to the minor release, whereas UCSC only lists the major release. My understanding is that all minor releases are guaranteed to share the same chromosome coordinates, and only represent local patches, but you guys surely know more about all of this. Just let me know about the best way forward. Florian On 26/07/16 21:27, "Obenchain, Valerie" <Valerie.Obenchain at RoswellPark.org> wrote:
Hi Florian, On 07/21/2016 01:47 AM, Hahne, Florian wrote:
This is a problem with the biomaRt package and its connection to the Ensembl archives, not Gviz. Here?s the call the fails: listMarts(host="feb2012.archive.ensembl.org", path="/biomart/martservice") It looks like Ensembl is no longer providing a download for the feb2012 archive. You could try the May2012 one, which according to this table (http://www.ensembl.org/info/website/archives/assembly.html) should still provide the mm9 (NCBIm37) genome: bm <- useMart(host = "may2012.archive.ensembl.org", biomart = "ENSEMBL_MART_ENSEMBL", dataset = "mmusculus_gene_ensembl") bmTrack <- BiomartGeneRegionTrack(start=26682683, end=26711643, chromosome=7, genome="mm9", biomart = bm) I?ll update the automated mapping from UCSC genome identifier to Biomart within Gviz, however I am more and more convinced that this whole setup is not ideal. I simply do not have the time to keep track of all the Ensembl changes and new genome versions. There really should be an annotation package or the like maintained by Bioconductor core or within the biomaRt package that gives a mapping from a UCSC genome identifier to an Ensembl genome version and the Ensembl archive to access that.
The mapping between genome identifiers seems like a natural fit for the GenomeInfoDb package. Mapping to a particular ensembl archive might be more appropriate to have in biomaRt but I'm open to what people think. If you're in favor of this re-org we can start incorporating the following into GenomeInfoDb: - Gviz:::.getBiobmart() # or move to biomaRt - Gviz:::.ucsc2Ensembl() - extdata/biomartVersionsNow.txt - extdata/biomartVersionsLatest.txt Any hints or tips for maintaining the .txt files, i.e., what worked, what you might do different the second time around? Valerie
Florian On 20/07/16 21:15, "Bioc-devel on behalf of James W. MacDonald" <bioc-devel-bounces at r-project.org on behalf of jmacdon at uw.edu> wrote:
Hi Holly, This list is intended for those that are developing packages. Your question should be asked on the support site (https://support.bioconductor.org). Please repost over there. Best, Jim On Wed, Jul 20, 2016 at 2:04 PM, Holly <xyang2 at uchicago.edu> wrote:
Dear Bioconductor helpers, I am trying to plot a region of interest using the Gviz package. I met error when running the following example code:
library(Gviz) library(GenomicRanges) bmTrack <- BiomartGeneRegionTrack(start=26682683, end=26711643,
+ chromosome=7, genome="mm9") Entity 'nbsp' not defined Entity 'hellip' not defined Entity 'hellip' not defined Entity 'nbsp' not defined Entity 'raquo' not defined Entity 'hellip' not defined Entity 'hellip' not defined Entity 'hellip' not defined Entity 'hellip' not defined Entity 'hellip' not defined Opening and ending tag mismatch: img line 68 and li Opening and ending tag mismatch: li line 68 and ul Opening and ending tag mismatch: ul line 67 and div Entity 'copy' not defined Opening and ending tag mismatch: div line 19 and body Opening and ending tag mismatch: body line 17 and html Premature end of data in tag html line 2 Error: 1: Entity 'nbsp' not defined 2: Entity 'hellip' not defined 3: Entity 'hellip' not defined 4: Entity 'nbsp' not defined 5: Entity 'raquo' not defined 6: Entity 'hellip' not defined 7: Entity 'hellip' not defined 8: Entity 'hellip' not defined 9: Entity 'hellip' not defined 10: Entity 'hellip' not defined 11: Opening and ending tag mismatch: img line 68 and li 12: Opening and ending tag mismatch: li line 68 and ul 13: Opening and ending tag mismatch: ul line 67 and div 14: Entity 'copy' not defined 15: Opening and ending tag mismatch: div line 19 and body 16: Opening and ending tag mismatch: body line 17 and html 17: Premature end of data in tag html line 2
sessionInfo()
R version 3.3.1 (2016-06-21) Platform: x86_64-w64-mingw32/x64 (64-bit) Running under: Windows 7 x64 (build 7601) Service Pack 1 locale: [1] LC_COLLATE=English_United States.1252 [2] LC_CTYPE=English_United States.1252 [3] LC_MONETARY=English_United States.1252 [4] LC_NUMERIC=C [5] LC_TIME=English_United States.1252 attached base packages: [1] grid parallel stats4 stats graphics grDevices utils [8] datasets methods base other attached packages: [1] Gviz_1.16.1 GenomicRanges_1.24.2 GenomeInfoDb_1.8.2 [4] IRanges_2.6.1 S4Vectors_0.10.2 BiocGenerics_0.18.0 loaded via a namespace (and not attached): [1] SummarizedExperiment_1.2.3 VariantAnnotation_1.18.3 [3] splines_3.3.1 lattice_0.20-33 [5] colorspace_1.2-6 htmltools_0.3.5 [7] rtracklayer_1.32.1 GenomicFeatures_1.24.4 [9] chron_2.3-47 interactiveDisplayBase_1.10.3 [11] survival_2.39-5 XML_3.98-1.4 [13] foreign_0.8-66 DBI_0.4-1 [15] ensembldb_1.4.7 BiocParallel_1.6.2 [17] RColorBrewer_1.1-2 matrixStats_0.50.2 [19] plyr_1.8.4 zlibbioc_1.18.0 [21] Biostrings_2.40.2 munsell_0.4.3 [23] gtable_0.2.0 latticeExtra_0.6-28 [25] Biobase_2.32.0 biomaRt_2.28.0 [27] BiocInstaller_1.22.3 httpuv_1.3.3 [29] AnnotationDbi_1.34.4 Rcpp_0.12.5 [31] acepack_1.3-3.3 xtable_1.8-2 [33] BSgenome_1.40.1 scales_0.4.0 [35] Hmisc_3.17-4 XVector_0.12.0 [37] mime_0.5 Rsamtools_1.24.0 [39] gridExtra_2.2.1 AnnotationHub_2.4.2 [41] ggplot2_2.1.0 digest_0.6.9 [43] biovizBase_1.20.0 shiny_0.13.2 [45] tools_3.3.1 bitops_1.0-6 [47] RCurl_1.95-4.8 RSQLite_1.0.0 [49] dichromat_2.0-0 Formula_1.2-1 [51] cluster_2.0.4 Matrix_1.2-6 [53] data.table_1.9.6 httr_1.2.1 [55] R6_2.1.2 rpart_4.1-10 [57] GenomicAlignments_1.8.4 nnet_7.3-12 Thank you for help, Holly
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