Hi, We have hybridizations done with the Affymetrix Arabidopsis Tiling chip. I have created a pd.at35b.mr.v04 package from the Affy BPMAP file using makePDpackage() from makePlatformDesign() and this works fine. However, The BPMAP is based on Genome Release TAIR5. In http://atensembl.arabidopsis.info/ the probe sequences of the tiling chip have been blasted against the TAIR7 release, and are available e.g. as GFF file or via a (local) ensemble installation. Is there a (convenient) way to create a PDpackage from these GFF data ? Any Code I could adapt ? Yours, Steffen
IPB Halle AG Massenspektrometrie & Bioinformatik Dr. Steffen Neumann http://www.IPB-Halle.DE Weinberg 3 http://msbi.bic-gh.de 06120 Halle Tel. +49 (0) 345 5582 - 1470 +49 (0) 345 5582 - 0 sneumann(at)IPB-Halle.DE Fax. +49 (0) 345 5582 - 1409