We have created a new experimental data package called 'seqc'. It includes gene-level read count data generated by the SEQC (SEquencing Quality Control) project, which is the third stage of the well-known MAQC project (a US FDA initiative). The SEQC/MAQC-III Consortium produced benchmark RNA-seq data for the assessment of RNA sequencing technologies and data analysis methods (published recently on Nature Biotechnology - http://www.ncbi.nlm.nih.gov/pubmed/25150838): Sequence reads were aligned to human reference genome hg19 using the Subread aligner and were then summarized to genes using the featureCounts program. This package includes the gene-level read count data for 2,758 libraries. It can be downloaded from the following link (188MB): http://bioconductor.org/packages/release/data/experiment/html/seqc.html In addition to the read count data, this package also includes exon-exon junction data generated for human brain reference RNA and universal human reference RNA samples. Exon-exon junctions were detected by using the Subjunc aligner. Moreover, TaqMan RT-PCR validation data for ~1000 genes and ERCC spike-in sequence data are included in this package as well. We hope this package is a useful resource for the community. Wei ______________________________________________________________________ The information in this email is confidential and intend...{{dropped:8}}
[Bioc-devel] Experimental data package 'seqc'
1 message · Wei Shi