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[Bioc-devel] proposal for additional seqlevelsStyle

4 messages · Vincent Carey, Hervé Pagès, Robert Castelo

Original
Vincent Carey
# 
I raised this issue previously with little response.

I'd propose that we add a column or two to genomeStyles()$Homo_sapiens
circular auto   sex NCBI UCSC dbSNP Ensembl

1    FALSE TRUE FALSE    1 chr1   ch1       1

2    FALSE TRUE FALSE    2 chr2   ch2       2


that includes the values for "NCBI reference sequence names"

See https://www.ncbi.nlm.nih.gov/nuccore/568815581 for one report on chr17,
and

https://www.ncbi.nlm.nih.gov/assembly/GCF_000001405.39

for a table that includes the Genbank labels.

Should I just file a PR at https://github.com/Bioconductor/GenomeInfoDb/ after
testing?
4 days later
Robert Castelo
# 
I second this, and would suggest to name the style as 'GRC' for "Genome 
Reference Consortium".

thanks Vince for bringing this up, being able to easily switch between 
genome styles is great.

if 'paste0()' in R is one of the most influential contributions to 
statistical computing

https://simplystatistics.org/2013/01/31/paste0-is-statistical-computings-most-influential-contribution-of-the-21st-century

i think that 'seqlevelsStyle()' from the GenomeInfoDb package is one of 
the most influential contributions to human genetics, if you think about 
the time invested by researchers in parsing and changing between 
different styles of chromosome names :)

robert.
On 06/12/2019 15:03, Vincent Carey wrote:
Hervé Pagès
# 
Hi Vince, Robert,

Looks like Vince wants the RefSeq accession e.g. NC_000017.11 for chrom 
17 in the GRCh38.

@Robert: Is this what you're also interested in?

The problem is that the RefSeq accessions are specific to a particular 
assembly (e.g. NC_000017.11 for chrom 17 in GRCh38 but NC_000017.10 for 
the same chrom in GRCh37).

Currently seqlevelsStyle() doesn't know how to distinguish between 
different assemblies of the same organism. Not saying it couldn't but it 
would require some thinking and some significant refactoring. It 
wouldn't be just a matter of adding a column to 
genomeStyles()$Homo_sapiens.

H.
On 12/10/19 14:19, Robert Castelo wrote:

  
    

      
      
    

  


  


      

    

    
1 day later

    

      
      

        


  

        

      Robert Castelo
    

    

      
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hi Herv?,

i didn't know about this new sequence style until Vince posted his 
message and we briefly talked about it at the European BioC meeting this 
week in Brussels. however, i didn't know that the style was specific to 
a particular assembly. i have no use case of this at the mome moment, 
i.e., i have not encountered myself any annotation or BAM file with 
chromosome names written that way, so i don't know how pressing this 
issue is, maybe Vince can tell us how spread such chromosome naming 
style may become in the near future.

naively, i'd think that it would be matter of adding a 
reference-specific column, i.e., 'GRCh38.p13', 'GRCh37.p13', etc., but i 
can imagine that maybe the "reference style" concept might not be the 
appropriate placeholder to map all different chromosome names of all 
different individual human genomes uploaded to NCBI. maybe we should 
wait until we have a specific use case .. Vince?

robert.

        
On 12/11/19 10:06 PM, Pages, Herve wrote: