Recoding variables based on reference values in data frame

Hello,

I'm not sure I understood, but try the following.


Kgeno <- read.table(text = "
SNP_ID SNP1 SNP2 SNP3 SNP4
Maj_Allele C G  C  A
Min_Allele T A T G
ID1 CC     GG     CT     AA
ID2 CC     GG     CC AA
ID3 CC     GG    nc    AA
ID4 _ _ _ _
ID5 CC     GG     CC     AA
ID6 CC     GG     CC     AA
ID7 CC     GG     CT     AA
ID8 _ _ _ _
ID9 CT     GG     CC AG
ID10 CC     GG     CC     AA
ID11 CC     GG     CT     AA
ID12 _ _ _ _
ID13 CC     GG     CC     AA
", header = TRUE, stringsAsFactors = FALSE)

dat

fun <- function(x){
     x[x %in% c("nc", "_")] <- NA
     MM <- paste0(x[1], x[1])  # Major Major
     Mm <- paste0(x[1], x[2])  # Major minor
     mm <- paste0(x[2], x[2])  # minor minor
     x[x == MM] <- 0
     x[x == Mm] <- 1
     x[x == mm] <- 2
     x
}

Kgeno[, -1] <- sapply(Kgeno[, -1], fun)
Kgeno


Also, the best way to post data is by using ?dput.

dput(head(Kgeno[, 1:5], 30))  # post the output of this


Hope this helps,

Rui Barradas

Em 02-07-2013 21:46, kathleen askland escreveu:

I'm new to R (previously used SAS primarily) and I have a genetics data
frame consisting of genotypes for each of 300+ subjects (ID1, ID2, ID3,
...) at 3000+ genetic locations (SNP1, SNP2, SNP3...). A small subset of
the data is shown below:
   SNP_ID SNP1 SNP2 SNP3 SNP4  Maj_Allele C G  C  A  Min_Allele T A T
G  ID1
CC     GG     CT     AA      ID2 CC     GG     CC AA      ID3 CC     GG
nc
AA      ID4 _ _ _ _  ID5 CC     GG     CC     AA      ID6 CC
GG     CC
      AA      ID7 CC     GG     CT     AA      ID8 _ _ _ _  ID9 CT     GG
CC AG      ID10 CC     GG     CC     AA      ID11 CC     GG     CT     AA
       ID12 _ _ _ _  ID13 CC     GG     CC     AA
The name of the data file is Kgeno.
What I would like to do is recode all of the genotype values to standard
integer notation, based on their values relative to the reference rows
(Maj_Allele and Min_Allele). Standard notation sums the total of minor
alleles in the genotype, so values can be 0, 1 or 2.

Here are the changes I want to make:
1. If the genotype= "nc" or '_" then set equal to NA.
2. If genotype value = a character string comprised of two consecutive
major allele values -- c(Maj_Allele, Maj_Allele) -- then set equal to 0.
3. If genotype  value= c(Maj_Allele, Min_Allele) then set equal to 1.
4. If genotype  value = c(Min_Allele, Min_Allele) then set equal to 2.

I've tried the following ifelse processing but get error (Warning:
Executed
script did not end with R session at the top-level prompt.  Top-level
state
will be restored) and can't seem to fix the code properly. I've
counted the
parentheses. Also, not sure if it would execute properly if I could
fix it.

# change 'nc' and '_' to NA, else leave as is:
Kgeno[,2] <- ifelse(Kgeno[,2] == "nc", "NA", Kgeno[,2])
Kgeno[,2] <- ifelse(Kgeno[,2] == "_", "NA", Kgeno[,2])

#convert genotype strings in the first data column to numeric values
#(two
major alleles=0, 1 minor and 1 major=1, 2 minor alleles=2), else
#leave as
is (to preserve NA values).

Kgeno[,2] <-

ifelse(Kgeno[,2] == noquote(paste(as.character(Kgeno[1,2]), as.character(
Kgeno[1,2]), sep=""), 0,

ifelse(Kgeno[,2] == noquote(paste(as.character(Kgeno[1,2]), as.character(
Kgeno[2,2]), sep=""), 1,

ifelse(Kgeno[,2] == noquote(paste(as.character(Kgeno[2,2]), as.character(
Kgeno[2,2]), sep=""), 2,
             Kgeno[,2])))


Finally, if above code were corrected, this would only change the first
column of data, but I would like to change all 3000+ columns in the same
way.

I would greatly appreciate some suggestions on how to proceed.

Thank you,

Kathleen

---
Kathleen Askland, MD
Assistant Professor
Department of Psychiatry & Human Behavior
The Warren Alpert School of Medicine
Brown University/Butler Hospital

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