Sorry!
In my experiment, there are two type of soil ( soil F and soil D), each half
of them were subjected to steam sterilize (result in FS and DS soil). A
equal volume of soil from two of the four soil types (F, D, FS, DS) were
mixed as follows: F+F, F+D, F+FS, F+DS, D+F, D+FS, D+DS, FS+DS (eight
treatment).
Two type of plant (A, B) were planted in the eight treatment of soil in pot.
There were 40 pots divided into 5 groups (8 pots for each group) for each
treatment*plant combination. Finally there were 80 groups for plant A and B
in total. The 40 groups for plant A were randomly arranged in plot 1, and
for plant B in plot 2.
The experiment were sampled for three times. In each sampling date, one pot
was randomly choosing from each group to measure biomass (80 pot for
sampling date).