LINEAR MIXED EFFECT

CAN ANYONE PLEASE HELP ME WITH THIS
i HAVE TO DO A MIXED EFFECT LINEAR MODEL WITH MY DATA DUE TO THE FACT THAT I
have pseudoreplication!
Although after reading and trying it for several times can get around due to
"Error in na.fail.default(list(date = c(1L, 2L, 3L, 4L, 5L, 6L, 7L, 8L,  :
missing values in object" 
I uploaded my data file
Thank you so much 
Kind regards 
AG
http://old.nabble.com/file/p26300394/rawoctobercalciumexperiment2.txt
rawoctobercalciumexperiment2.txt

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