Message-ID: <200505252240.j4PMeD0Y028347@faraday.gene.com>
Date: 2005-05-25T22:40:13Z
From: Bert Gunter
Subject: global normalization
In-Reply-To: <200505252233.j4PMX5Z04111@webmail13.cac.psu.edu>
Please address this question to the appropriate BioConductor or other
microarray list (or to the limma package author, as the Posting Guide asks).
It is not appropriate for R-Help.
-- Bert Gunter
Genentech Non-Clinical Statistics
South San Francisco, CA
"The business of the statistician is to catalyze the scientific learning
process." - George E. P. Box
> -----Original Message-----
> From: r-help-bounces at stat.math.ethz.ch
> [mailto:r-help-bounces at stat.math.ethz.ch] On Behalf Of
> NATALIA F TCHETCHERINA
> Sent: Wednesday, May 25, 2005 3:33 PM
> To: r-help at stat.math.ethz.ch
> Subject: [R] global normalization
>
> Hello all,
> I have question about global normalization.
> I have data from big experement(two-color cDNA arrays) where
> used three
> different layouts but the same set of genes.
> I used limma package for within array normalization.
> My question is: how I can do between array normalization
> (global) if arrays have
> different layout(different location and different number
> technical replicates
> (number of spots of a gene on an array))?
>
> Sincerely, Natalia.
>