Message-ID: <6510f373.31ba.1481192d74f.Coremail.my1stbox@163.com>
Date: 2014-08-26T09:08:07Z
From: my1stbox
Subject: What would a typical miRNA microarray analysis workflow look like?
Hi all,
What would a typical miRNA microarray analysis workflow look like? Say just a test group of 5 replicates from bladder cancer tumor and corresponding control group from normal tissue of the same patients. What could I do to make the analysis seems more sophisticated. I have done differential expression analysis, target gene prediction, and GO, pathway enrichments of target genes. What else could I do? It would be better if you can specify some packages or codes.
Regards,
Allen
PS: Is paired (t)-test necessery? I found that DE gene number from paired t-test is about half of that from normal limma functions( which I don't know how to do paired test if it could).
2014-08-26
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