Hello all, I have question about global normalization. I have data from big experement(two-color cDNA arrays) where used three different layouts but the same set of genes. I used limma package for within array normalization. My question is: how I can do between array normalization (global) if arrays have different layout(different location and different number technical replicates (number of spots of a gene on an array))? Sincerely, Natalia.
global normalization
2 messages · NATALIA F TCHETCHERINA, Bert Gunter
Please address this question to the appropriate BioConductor or other microarray list (or to the limma package author, as the Posting Guide asks). It is not appropriate for R-Help. -- Bert Gunter Genentech Non-Clinical Statistics South San Francisco, CA "The business of the statistician is to catalyze the scientific learning process." - George E. P. Box
-----Original Message----- From: r-help-bounces at stat.math.ethz.ch [mailto:r-help-bounces at stat.math.ethz.ch] On Behalf Of NATALIA F TCHETCHERINA Sent: Wednesday, May 25, 2005 3:33 PM To: r-help at stat.math.ethz.ch Subject: [R] global normalization Hello all, I have question about global normalization. I have data from big experement(two-color cDNA arrays) where used three different layouts but the same set of genes. I used limma package for within array normalization. My question is: how I can do between array normalization (global) if arrays have different layout(different location and different number technical replicates (number of spots of a gene on an array))? Sincerely, Natalia.