ANOVA in R

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I think you start by doing your homework:

1. Read "An Inroduction to R" (ships with R) or other R online
tutorial. There are many good ones.

2. Use R's Help system:
?aov
?lm
?anova

there will be relevant links in these docs that you should follow,
especially to the use of formulas for model specification and
summary() for printing results (including anova tables).

3. Alternatively, have a look at the Rcmdr package for a GUI interface
to R that may meet your needs while allowing you to skip the homework.

Cheers,
Bert
ID

a_t1    a_t2    b_t1    b_t2
CACCCGTAGAACCGACCTTGCG_mmu-miR-99b-5p    1578    1941    2348    10941
CACCCGTAGAACCGACCTTGC_mmu-miR-99b-5p    442    426    564    3839
CACCCGTAGAACCGACCTTG_mmu-miR-99b-5p    54    43    66    253
CCGTAGAACCGACCTTGCG_mmu-miR-99b-5p    26    33    33    157
CGTAGAACCGACCTTGCG_mmu-miR-99b-5p    23    22    38    187
CACCCGTAGAACCGACCT_mmu-miR-99b-5p    17    27    31    189
ACCCGTAGAACCGACCTTGCG_mmu-miR-99b-5p    14    19    30    94

I want to run an ANOVA test on the rows above. I have two conditions with
two replicates each. I assume that there is no difference between a and b
for the different rows. I want to get which rows a different between a and
b.
How do I approach this in R?

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Bert Gunter
Genentech Nonclinical Biostatistics

(650) 467-7374