You probably need specify the repeated measures by using an "Error" term in aov for repeated measures: aov(trait ~ species + strain + Error(species/strain)) Take a look at Ripley's book. Treat above with caution: I am no expert, but the answer is in that direction... Michael Jerosch-Herold I have a question on using R to analyze data with repeated measurements. I have 2 species with several strains (12) per species, each of which has been measured twice with for a given trait. No particular covariance, just two measures. Now I want to analyze the data with an ANOVA (aov) considering these repeated measures to get the MSq and SSq for the species and strain level. I would like to know how to write the ANOVA model in R. I have done the following: aov(trait ~ species + strain/replicate) Is it accurate? Thanks a lot, Christian
AOV with repeated measures
1 message · Michael Jerosch-Herold