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SAMseq errors

3 messages · array chip, Jeff Newmiller

Original
array chip
# 
Hi, I am trying to using SAMseq() to analyze my RNA-seq experiment (20000 genes x 550 samples) with survival endpoint. It quickly give the following error:
Attaching package: ?matrixStats?
The following objects are masked from ?package:Biobase?:
? ? anyMissing, rowMedians
Warning messages:1: package ?samr? was built under R version 3.3.3?2: package ?matrixStats? was built under R version 3.3.3
Estimating sequencing depths...Error in quantile.default(prop, c(0.25, 0.75)) :?? missing values and NaN's not allowed if 'na.rm' is FALSEIn addition: Warning message:In sum(x) : integer overflow - use sum(as.numeric(.))Error during wrapup: cannot open the connection
locale:[1] LC_COLLATE=English_United States.1252? LC_CTYPE=English_United States.1252? ? LC_MONETARY=English_United States.1252[4] LC_NUMERIC=C? ? ? ? ? ? ? ? ? ? ? ? ? ?LC_TIME=English_United States.1252? ??
attached base packages:[1] stats? ? ?graphics? grDevices datasets? utils? ? ?methods? ?base? ? ?
other attached packages:[1] samr_2.0? ? ? ? ? ? ?matrixStats_0.52.2? ?impute_1.48.0? ? ? ? BiocInstaller_1.24.0 rcom_3.1-3? ? ? ? ? ?rscproxy_2.1-1? ? ??
loaded via a namespace (and not attached):[1] tools_3.3.2

I checked, my data matrix and y variables have no missing values. Anyone has suggestions what's going on?
Thank you!
John
array chip
# 
Sorry forgot to use plain text format, hope this time it works:

Hi, I am trying to using SAMseq() to analyze my RNA-seq experiment (20000 genes x 550 samples) with survival endpoint. It quickly give the following error:
Loading required package: impute
Loading required package: matrixStats

Attaching package: ?matrixStats?

The following objects are masked from ?package:Biobase?:

? ? anyMissing, rowMedians

Warning messages:
1: package ?samr? was built under R version 3.3.3?
2: package ?matrixStats? was built under R version 3.3.3
Estimating sequencing depths...
Error in quantile.default(prop, c(0.25, 0.75)) :?
? missing values and NaN's not allowed if 'na.rm' is FALSE
In addition: Warning message:
In sum(x) : integer overflow - use sum(as.numeric(.))
Error during wrapup: cannot open the connection
R version 3.3.2 (2016-10-31)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows 7 x64 (build 7601) Service Pack 1

locale:
[1] LC_COLLATE=English_United States.1252? LC_CTYPE=English_United States.1252? ? LC_MONETARY=English_United States.1252
[4] LC_NUMERIC=C? ? ? ? ? ? ? ? ? ? ? ? ? ?LC_TIME=English_United States.1252? ??

attached base packages:
[1] stats? ? ?graphics? grDevices datasets? utils? ? ?methods? ?base? ? ?

other attached packages:
[1] samr_2.0? ? ? ? ? ? ?matrixStats_0.52.2? ?impute_1.48.0? ? ? ? BiocInstaller_1.24.0 rcom_3.1-3? ? ? ? ? ?rscproxy_2.1-1? ? ??

loaded via a namespace (and not attached):
[1] tools_3.3.2


I checked, my data matrix and y variables have no missing values. Anyone has suggestions what's going on?

Thank you!

John
Jeff Newmiller
# 
A) This list is a general interest list on the R language... you have posed your question as if you were looking for domain experts such as you might be more likely to find on the Bioconductor mailing list. 

B) Example is not reproducible. [1][2][3]

C) Just because your data don't have missing values does not mean that your early analysis steps don't create them, e.g. by taking the logarithm of negative numbers. Look at intermediate values in your analysis, and read the documentation for steps you are treating as "magic black boxes".

[1] http://stackoverflow.com/questions/5963269/how-to-make-a-great-r-reproducible-example

[2] http://adv-r.had.co.nz/Reproducibility.html

[3] https://cran.r-project.org/web/packages/reprex/index.html (read the vignette)