mutually exclusive events

Hi:

I am trying to identify mutually exclusive events from the following
example:

In cluster 1 :  G1, G2, G5 are mutated

In cluster 2:    G2, G3, G5 are mutated.


I am interested in finding such G2-G5 event and G1-G3 events.

In total I have a 8 clusters and 150 gene (1200 rows x 4 columns).

What test could be appropriate to identify such pairs.

In my naive understanding would a fishers-exact test give such
combinations.

Thanks a lot.

-Adrian

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David?s answer assumes a more complicated objective, but obviously we are both unclear as to what you want.  Are you trying to find out which clusters have a unique pattern of mutation? (probably all of them, with so few clusters and so many genes?)

For either objective, this is not a statistical test, but a problem of identification.  For the simpler question, create a data frame with each row being the 150 1s and 0s associated with each cluster, and use duplicated() to identify unique rows. (unique rows will return ?FALSE?)

Untested

On Aug 2, 2014, at 11:41 AM, David Winsemius <dwinsemius at comcast.net> wrote:

On Aug 2, 2014, at 11:11 AM, Adrian Johnson wrote:

Hi:

I am trying to identify mutually exclusive events from the following
example:

#-------------
dat <- read.table(text="Cluster      Gene      Mutated    not_mutated
 1             G1             1              0
 1             G2             1              0
 1             G3             0              1
 1             G4             0              1
 1             G5             1              0
 2             G1             0              1
 2             G2             1              0
 2             G3             1              0
 2             G4             0              0
 2             G5             1              0", header=TRUE, stringsAsFactors=FALSE)

with(dat, table(Cluster, Gene, Mutated)  )
#----------------
, , Mutated = 0

      Gene
Cluster G1 G2 G3 G4 G5
     1  0  0  1  1  0
     2  1  0  0  1  0

, , Mutated = 1

      Gene
Cluster G1 G2 G3 G4 G5
     1  1  1  0  0  1
     2  0  1  1  0  1
#--------------
Or:
xtabs(Mutated ~ Cluster+Gene, data=dat)
#----------------
      Gene
Cluster G1 G2 G3 G4 G5
     1  1  1  0  0  1
     2  0  1  1  0  1


I'm a bit unclear about your goals. Are you trying to identify the "Gene"s that have only one "Cluster" mutated as the "G1-G3" events and the Gene's that have either-Cluster but not both as the "G2-G5" events?

If so you can choose the columns that have a sum of 2 for the first and columns with sum of 1 for the second.

In cluster 1 :  G1, G2, G5 are mutated

In cluster 2:    G2, G3, G5 are mutated.


I am interested in finding such G2-G5 event and G1-G3 events.

In total I have a 8 clusters and 150 gene (1200 rows x 4 columns).

What test could be appropriate to identify such pairs.

In my naive understanding would a fishers-exact test give such
combinations.

It's even less clear what sort of "test" you propose. `fisher.test` is a test of association. It doesn't identify combinations.

Thanks a lot.

-Adrian

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David Winsemius
Alameda, CA, USA

Don McKenzie
Research Ecologist
Pacific Wildland Fire Sciences Lab
US Forest Service

Affiliate Professor
School of Environmental and Forest Sciences
University of Washington
dmck at uw.edu





        [[alternative HTML version deleted]]

Hi:

I am trying to identify mutually exclusive events from the following
example:


Cluster      Gene      Mutated    not-mutated
  1             G1             1              0
  1             G2             1              0
  1             G3             0              1
  1             G4             0              1
  1             G5             1              0
  2             G1             0              1
  2             G2             1              0
  2             G3             1              0
  2             G4             0              0
  2             G5             1              0


In cluster 1 :  G1, G2, G5 are mutated

In cluster 2:    G2, G3, G5 are mutated.


I am interested in finding such G2-G5 event and G1-G3 events.

In total I have a 8 clusters and 150 gene (1200 rows x 4 columns).

What test could be appropriate to identify such pairs.

In my naive understanding would a fishers-exact test give such
combinations.

Thanks a lot.

-Adrian