Hi all, I am interested in plotting a heatmap of a set of genes. I would like the text labels of these genes to be colored red rather than black if they meet certain statistical criteria (using an if statement). I'm not sure how to change individual color labels without changing them all. Can anyone provide some insight on how to do this? Thanks in advance, Jake
conditional coloring of image labels
2 messages · Jake Michaelson
On Friday 11 November 2005 10:37 am, Jake Michaelson wrote:
I'll clarify a little and hopefully this will make more sense (thanks for the friendly encouragement): Let's say I have 6 samples and am looking at 3 genes, with intensities in a matrix as follows:
genes=cbind(ABC1=c(3,4,4,5,6,3), ABC2=c(4,3,4,7,7,8), ABC3=c(8,7,8,6,3,2)) genes
ABC1 ABC2 ABC3 [1,] 3 4 8 [2,] 4 3 7 [3,] 4 4 8 [4,] 5 7 6 [5,] 6 7 3 [6,] 3 8 2 ###plot the image
image(1:nrow(genes), 1:ncol(genes), genes, axes = FALSE, xlab = "", ylab =
"", col=cm.colors(256)) ###label the axes
axis(1, 1:nrow(genes), labels = rownames(genes), las = 2, line = -0.5, tick =
0)
axis(2, 1:ncol(genes), labels = colnames(genes), las = 2, line = -0.5, tick =
0) Now let's say (I'm just making these numbers and the scenario up here -- for simplicity's sake) that I had run a statistical analysis previously and wanted to label the genes that showed a significance of p< 0.05. Let's say that ABC1 and ABC3 had p<0.05 (assume that these values would be in a two-column matrix with the gene name and its p-value).
sig=cbind(name=c("ABC1", "ABC2", "ABC3"), pvalue=c(0.005, 0.1, 0.001))
sig
name pvalue [1,] "ABC1" "0.005" [2,] "ABC2" "0.1" [3,] "ABC3" "0.001" I now want these (the names of the significant genes) to be labeled in red rather than black on the plot. I would eventually write a script that would generate a large number of these images, each with a different set of genes. I would like to insert some sort of conditional formatting so that if that gene meets the significance threshold, the name is automatically plotted in red on the plot. I hope this is more clear and effective in explaining what I'm looking for. Thanks, --Jake
Hi all, I am interested in plotting a heatmap of a set of genes. I would like the text labels of these genes to be colored red rather than black if they meet certain statistical criteria (using an if statement). I'm not sure how to change individual color labels without changing them all. Can anyone provide some insight on how to do this? Thanks in advance, Jake
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