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Today's Topics:
1. Doing repeated measures on a randomized block design
(Richard Boyce)
----------------------------------------------------------------------
Message: 1
Date: Fri, 14 Jun 2019 18:41:47 +0000
From: Richard Boyce <boycer at nku.edu>
To: "r-sig-ecology at r-project.org" <r-sig-ecology at r-project.org>
Subject: [R-sig-eco] Doing repeated measures on a randomized block
design
Message-ID: <0B581062-D8DA-435D-87D0-BC99FED2D151 at nku.edu>
Content-Type: text/plain; charset="utf-8"
I?m measuring chlorophyll fluorescence (FvFm), my measured variable, on N and S exposures (treatment variable) of 4 red cedar trees. Here?s what the beginning of the data file looks like:
head(perm.fvfm).
Tree Exposure Date FvFm
1 1 S 13.Feb 0.775
2 1 N 13.Feb 0.795
3 2 S 13.Feb 0.737
4 2 N 13.Feb 0.759
5 3 S 13.Feb 0.615
6 3 N 13.Feb 0.712
If I were just doing this one time, this would be a randomized block design, where trees were the blocks (random variable) and exposure was the treatment variable (fixed variable). Actually, since there are only two treatment levels, it would be a paired t-test.
However, I?ve repeated this on many dates (18 so far this year). So this also requires a repeated-measures design, with trees as subjects.
Repeated-measures, however, usually have time (date) as a within-subject variable and then some other treatment that is a between-subjects variable. I don?t have have a between-subjects variable, however, as all subjects (trees) get both levels of exposure and all levels of time (date).
I?ve searched the web, but there is not a lot out there for this kind of design. It looks like lm, lme, lmer, and permuco in R might all work, but advice for how to set up the Error() or random variable designations are confusing and sometimes contradictory. Any advice would be much appreciated!
Thanks,
Rick Boyce
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