Message-ID: <637b5466-8e4b-0e92-fc24-432612ade2a7@urjc.es>
Date: 2018-10-24T08:58:44Z
From: Marcelino de la Cruz Rot
Subject: 2-way adonis (PERMANOVA) incl interaction - how to test for main effects?
In-Reply-To: <CANW5OGD0yRr8mUcu9MVrF6YNsudZdPRPbN0BMvyiV+RoFjLaAQ@mail.gmail.com>
El 24/10/2018 a las 10:29, Ellen Pape escribi?:
> Hello,
>
> Yes, but is there no way of testing for the main effects and
>
> > interaction simultaneously?
> In general, this is "not allowed", to say it with your words.
>
> OK, but this is done in general ANOVAs, no? test for interaction and
> main effects simultaneously (and then if you have a sign interaction
> term, you cannot interpret the tests for the main effects) - at least
> that is how I was taught how to do this (but maybe this is wrong?)
You are completely right. Maybe I? misunderstood you as, in my opinion,
the tests is for the interaction only (even with the main effects
included in the model), not "for the? main effects and the interaction
simultaneously".
>
> FYI - package "RVAideMemoire" does indeed contain a function to
> perform type II adonis tests ("adonis.II"), but no type III tests..
Interesting. But this is? the same as adonis2(formula, by="margin"),
isn't it?
Cheers,
Marcelino
>
> best wishes to all,
> Ellen
> On Tue, 16 Oct 2018 at 12:24, Marcelino De La Cruz Rot
> <marcelino.delacruz at urjc.es <mailto:marcelino.delacruz at urjc.es>> wrote:
>
> El 16/10/2018 a las 12:09, Ellen Pape escribi?:
> > Hi,
> >
> > Yes, but is there no way of testing for the main effects and
> > interaction simultaneously?
>
> In general, this is "not allowed", to say it with your words.
>
> >
> > I also thought of first testing the interaction:
> > adonis2(speciesdataset~ A *** B, by="margin") , and then - if the
> > interaction is not significant- testing for the main effects by
> > adonis2(speciesdataset~ A *+* B, by="margin") . Is this "allowed"?
>
> This is analogous to, e.g., an ANCOVA, isn't it? Therefore it makes
> quite sense.
>
>
> >
> > Thanks,
> > Ellen
> >
> > On Tue, 16 Oct 2018 at 12:01, Marcelino De La Cruz Rot
> > <marcelino.delacruz at urjc.es <mailto:marcelino.delacruz at urjc.es>
> <mailto:marcelino.delacruz at urjc.es
> <mailto:marcelino.delacruz at urjc.es>>> wrote:
> >
> >? ? ?El 16/10/2018 a las 11:51, Ellen Pape escribi?:
> >? ? ?> Hi all,
> >? ? ?>
> >? ? ?Maybe adonis2(speciesdataset~ A + B, by="margin") ?
> >
> >
> >
> >? ? ?> I don't know whether this is the correct mailing group to
> >? ? ?address this
> >? ? ?> question:
> >? ? ?>
> >? ? ?> I would like to perform a 2-way permanova analysis in R (using
> >? ? ?adonis in
> >? ? ?> vegan). By default you are performing sequential tests
> >? ? ?(by="terms"), so
> >? ? ?> when you have 2 or more factors, the order of these
> factors matter.
> >? ? ?> However, since I wanted to circumvent this, I chose for
> the option
> >? ? ?> by="margin" (adonis2(speciesdataset~A*B, by="margin")) but
> then
> >? ? ?only the
> >? ? ?> effect of the interaction is tested. On the "help page" of
> >? ? ?anova. cca it
> >? ? ?> says: "if you select by="margin" -> the current function only
> >? ? ?evaluates
> >? ? ?> marginal terms. It will, for instance, ignore main effects
> that are
> >? ? ?> included in interaction terms."
> >? ? ?>
> >? ? ?>
> >? ? ?> My question now is: can I somehow get the main effects tested
> >? ? ?anyhow, when
> >? ? ?> the interaction term is not significant?
> >? ? ?>
> >? ? ?> Thanks,
> >? ? ?> Ellen
> >? ? ?>
> >? ? ?>? ? ? ?[[alternative HTML version deleted]]
> >? ? ?>
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> >
> >? ? ?--
> >? ? ?Marcelino de la Cruz Rot
> >? ? ?Depto. de Biolog?a y Geolog?a
> >? ? ?F?sica y Qu?mica Inorg?nica
> >? ? ?Universidad Rey Juan Carlos
> >? ? ?M?stoles Espa?a
> >
>
> --
> Marcelino de la Cruz Rot
> Depto. de Biolog?a y Geolog?a
> F?sica y Qu?mica Inorg?nica
> Universidad Rey Juan Carlos
> M?stoles Espa?a
>
--
Marcelino de la Cruz Rot
Depto. de Biolog?a y Geolog?a
F?sica y Qu?mica Inorg?nica
Universidad Rey Juan Carlos
M?stoles Espa?a