multiple factors in vegan:betadisper?
Dear Cameron,
A while ago, I had asked a similar question to Marti Anderson. Here's is
what she had replied:
"I have actually moved away from doing 2-way PERMDISP types of
analyses. The reason for this is that there are inherent
difficulties in testing homogeneity of dispersions across the
main effects in cases where there is an interaction between the
two factors in their *locations*, when tested using PERMANOVA.
Think of it this way - suppose there is no differences in the
locations of samples in multivariate space among the levels of
factor A and in level 1 of factor B, but there are such
differences (once again, in *location*) in level 2 of factor B
(which would correspond to an interaction in PERMANOVA). Then,
clearly, this would be detected as a difference in dispersion,
even though it is entirely due to the locations of cells
(combinations of factors) in the two-way design. Thus, it seems
to me that for the two-way design, to test dispersions, one can:
(i) test for differences in dispersion among the individual axb
cells (which is simply a one-way test where the individual cells
are treated as levels of a single factor) and
(ii) test for an interaction in a two-way PERMANOVA and if this
is NS, then one can examine the test of dispersions (or
locations, for that matter) separately and independently for the
two factors."
Hope this helps.
Cheers
Etienne
Le mardi 21 septembre 2010 ? 13:24 -0700, Cameron N. Carlyle a ?crit :
Dear all:
I am wondering if it is possible to implement multiple factors in betadisper
{vegan} when trying to run a Permutational Analysis of multivariate
dispersions (PERMDISP)? To run a PERMANOVA using adonis you are able to
enter a formula, but the usage of betadisper seems to only allow a single
group.
If it isn't possible, does anybody know a work around?
Thank you,
Cam
--
Cameron N. Carlyle
PhD Candidate
Botany,
University of British Columbia
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Etienne Lalibert? ================================ School of Forestry University of Canterbury Private Bag 4800 Christchurch 8140, New Zealand Phone: +64 3 366 7001 ext. 8365 Fax: +64 3 364 2124 www.elaliberte.info >From October 11: School of Plant Biology The University of Western Australia 35 Stirling Highway Crawley, Perth Western Australia 6009