Redundancy analysis with ONLY factor variables
Dear list, I wonder if RDA (and variance partitioning) is valid if the species table is regressed against three factor variables only. Factor1 has three levels, the other two Factors have two levels, resulting in a design with 12 different level combinations (nearly balanced). The varpart would then look like varpart (species, Factor1, Factor2, Factor 3). I am asking because if i repeat the RDA with a combined factor: comb <- paste(Factor1, Factor2, Factor3) rda(species~comb). i get almost the exact same result as with rda(species ~ Factor1+Factor2+Factor3). In both cases, i get the same horseshoe-like ordination (showing a very strong separation on axis 1), but different sets of biplot arrows. With varpart, no joint explained variability is found, and interestingly the ratio between the single explained fractions resembles the ratios between the F-statistics obtained with PERMANOVA (adonis2(species~Factor1+Factor2+Factor3)). Ecologically, the results do make a lot of sense. I was wondering if someone could explain if the approach is valid, if joint explained variability can be found with such a design, and if there are pitfalls one needs to take care of. Thank you and best wishes, Tim
Dr. Tim Richter-Heitmann University of Bremen Microbial Ecophysiology Group (AG Friedrich) FB02 - Biologie/Chemie Leobener Stra?e (NW2 A2130) D-28359 Bremen Tel.: 0049(0)421 218-63062 Fax: 0049(0)421 218-63069