Mixed Models in a very basic replication design
Dear Group, i am to plan a very basic factorial greenhouse experiment, and this time i will first ask for statistical advise before execution :). It will encompass two treatment types with two levels each, two sampling dates with five replicates each, resulting in 2 x 2 x 2 x 5 = 40 samples. I guess, this is a basic three way ANOVA (~ Treatment A * Treatment B * Time). However, the arrangement of the replicates in the greenhouse will be randomized. I have only a limited understanding of mixed models, obviously, but does the randomized location of the plant also requires the introduction of a random effect (~ Treatment A * Treatment B * Time + (1|Location)?. How do i best code location in this case? Thank you!
Tim Richter-Heitmann University of Bremen Microbial Ecophysiology Group (AG Friedrich) FB02 - Biologie/Chemie Leobener Stra?e (NW2 A2130) D-28359 Bremen Tel.: 0049(0)421 218-63062 Fax: 0049(0)421 218-63069