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Macroecology of sexual selection

Hi, Bolker, nice to meet you. 
I?m doing my master degree at University of S?o Paulo. I?m traing to analize how the temperature affects the sexual traits. I have a morfological data from 21 populations along to east cost of Brazil. For each population I have just one data of temperture and one coordenates (Lat, Lon). And I want see the temperatura efect in male and females (like an ANCOVA in a simple case). So, I don?t know what is kind of analise that I need to do, because, all that I tried to do, result in the same problem: 


"Error in getCovariate.corSpatial(object, data = data) : 
cannot have zero distances in "corSpatial" ". 


Plesae, I'd greatly appreciate advice on the matter. 


A hug, Soly