[With apologies, I am re-forwarding this to r-sig-mixed-models: I prefer to keep discussions there so they can be publicly viewed/archived.]
On 14-01-08 11:34 AM, jersa at centrum.cz wrote:
Dear Ben, thank you so much for your help. It is rather hard for me to imagine all the statistical consequencies when building this model. Basically I do not see any clear pattern in the data, it seems that mother vicinity has no effect on germination and germination is quite random. I only see that the second year yielded most germinations, which is also confirmed in the tests.
OK
The set up of directions was in all plants same (N, SE and SW).
So there would be at least some possibility of consistent effects of direction across plants (as well as random variations in those effects across plants)
I tried to fit both variants you suggested (see bellow). And the results are nearly identical. May I aks one more question about plotting the probability to germinate with distance? Which function would you recommend for this?
It's probably best to construct a new data frame that has the desired distance vector in it, e.g. newdat <- data.frame(distance=seq(100)) then use predict() to get the probability newdat$germ_prob <- predict(model,newdata=newdat,type="response")
Thank you very much and have a nice day Jana
prot1<-glmer(germination~distance+year+(1|plant/direction),family=binomial,data=prot)
summary(prot1) Random effects: Groups Name Variance Std.Dev. direction:plant (Intercept) 0.0544 0.2332 plant (Intercept) 1.4151 1.1896 Number of obs: 252, groups: direction:plant, 21; plant, 7
I thought you said you had 10 plants, and I would have inferred 360 observations from your experimental design -- actually only 7 / 252?
Fixed effects: Estimate Std. Error z value Pr(>|z|)
(Intercept) -1.840153 0.617722 -2.979 0.00289 ** vzd -0.004394 0.005174 -0.849 0.39572 year2009 0.995211 0.434851 2.289 0.02210 * year2011 0.588760 0.444732 1.324 0.18555 Treating distance (I assume that's vzd?) as continuous is a good idea, although you should take a look at the data to see that there isn't a strong nonlinear trend.
prot2<-glmer(germination~distance+year+(distance|plant),family=binomial,data=prot)
summary(prot2)
Random effects: Groups Name Variance Std.Dev. Corr
kytka (Intercept) 7.580e-01 0.870637
vzd 4.647e-05 0.006817 1.00
Number of obs: 252, groups: kytka, 7
Here 'kytka' is plant? The distance effect and intercept are perfectly correlated here, suggesting overfitting -- and the distance variance is extremely small (ditto) -- yo
Fixed effects: Estimate Std. Error z value Pr(>|z|)
(Intercept) -1.707002 0.527848 -3.234 0.00122 ** vzd -0.007350 0.005982 -1.229 0.21916 year2009 0.993268 0.435158 2.283 0.02246 * year2011 0.587586 0.444938 1.321 0.18663 Notice the fixed-effect estimates here are nearly identical to the previous case, because the estimates for the variances of the terms you added to the model are nearly zero.